Transfection Protocol for Primary Hepatocytes ( Rat Hepatocytes, Mouse Hepatocytes and Human Hepatocytes) in Targefect Handbook of Transfection Protocols

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation.

We present a detailed protocol for the transient transfection of non-proliferating primary cultured hepatocytes that is easily reproducible. Using a modification of the classical CaPO4/DNA precipitation method, this protocol is an inexpensive alternative to other methods that are often cumbersome, expensive, difficult to reproduce or harmful to primary hepatocytes. Because only 0.5 x 10(6) cell...

متن کامل

Metabolism and Cytotoxic Mechanisms of Nitroglycerin in Isolated Rat Hepatocytes

     It has been proposed that organic nitrates such as glyceryl trinitrate (GTN), used in the treatment of cardiovascular diseases, act by producing nitric oxide (NO). However, the biochemical pathway for NO formation from GTN is not well understood. In the present study, we showed that nitrate formation from GTN, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione w...

متن کامل

Lipid-mediated transfection of normal adult human hepatocytes in primary culture.

The aim of this work was to develop a procedure for the lipid-mediated transfection of DNA into normal adult human hepatocytes in culture. Cells were plated in a serum-free culture medium at various cell densities, on plastic or collagen-coated dishes, both in the absence and in the presence of epidermal growth factor (EGF). The cells were incubated for various periods of time with mixtures of ...

متن کامل

Cholesterol Uptake in Cultured Primary Mouse Hepatocytes

1 Intracellular Cholesterol Binding Proteins Enhance HDL-mediated 2 Cholesterol Uptake in Cultured Primary Mouse Hepatocytes 3 By 4 5 Stephen M. Storey, Avery L. McIntosh, Huan Huang, Kerstin K. Landrock, Gregory G. 6 Martin, Danilo Landrock, H. Ross Payne, Barbara P. Atshaves, Ann B. Kier, and Friedhelm 7 Schroeder 8 9 from the 10 Departments of Physiology and Pharmacology and Pathobiology, 11...

متن کامل

3D Cultivation Techniques for Primary Human Hepatocytes

One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions result in a loss of hepatocyte morphology and of liver-specific functions, such as drug-metabolizing enzymes and transporters. As it has been demonstrat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Protocol Exchange

سال: 2011

ISSN: 2043-0116

DOI: 10.1038/protex.2011.226